Highly sensitive detection of methamphetamine (stimulant drug) by using SPR-based immunosensor and kinetic study of the immunoreaction on sensor element

• Nakata Seiji

  Kyushu University

• Sakai Go

  Kyushu University

• Uda Taizo

  Hiroshima Prefectural University

• Miura Norio

  Kyushu University

• Yamazoe Noboru

  Kyushu University

Bibliographic Information

Other Title

• SPR免疫センサによるメタンフェタミン(覚醒剤)の高感度検出とその抗原抗体反応解析
• SPR メンエキ センサ ニ ヨル メタンフェタミン カクセイザイ ノ コウカンド ケンシュツ ト ソノ コウゲン コウタイ ハンノウ カイセキ

Abstract

Highly sensitive detection of methamphetamine (MA) was realized by using an immunosensor based on surface plasmon resonance (SPR) phenomena. A methamphetamine-bovine serum albumin (MA-BSA) conjugate was prepared as an antigen, and it was immobilized on the Au thin film of sensor-chip. Introduction of MA into the antibody solution was found to decrease the incident angle shift sharply because of the inhibition effect of MA. Association constants between anti-MA antibody and MA as well as anti-MA antibody and MA-BSA were evaluated from the Langmuir isotherm adsorption model. The association constants between anti-MA antibody and MA or MA-BSA were estimated as 5.68×10⁶M^-1, 2.92×10⁷ M^-1 respectively. The association rate constant and dissociation rate constant were also estimated from typical transient responses to antibody solutions.

Journal

• IEEJ Transactions on Sensors and Micromachines

  IEEJ Transactions on Sensors and Micromachines 119 (11), 581-586, 1999

  The Institute of Electrical Engineers of Japan

Keywords

• SPR

Details

• CRID: 1390001204462609024
• NII Article ID: 10013062752
• NII Article ID: 10004834619
• NII Book ID: AN1052634X
• DOI: 10.1541/ieejsmas.119.581
• ISSN: 13475525
• ISSN: 13418939
• NDL BIB ID: 4892822
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4892822
• Web Site: http://www.jstage.jst.go.jp/article/ieejsmas1995/119/11/119_11_581/_pdf
• Data Source:
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  • NDL
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  • CiNii Articles
• Abstract License Flag: Disallowed