集積化微小酵素センサ構築のための酸化酵素-ペルオキシダーゼ同時固定化電極の特性評価 Characterization of the oxidase-peroxidase electrode for the integrated miniature enzyme electrodes
The oxidase-peroxidase (HRP) co-immobilized electrode was characterized from the standpoint of an integrated miniature enzyme electrode development for diagnostic applications. The sensor characteristics were examined in terms of (1) contribution of enzyme immobilization materials, (2) use of other oxidases than glucose oxidase (GOD), and (3) control of enzyme sensor responses by controlling an electro-conductivity of an enzyme immobilized membrane. By comparing responses of GOD-HRP electrodes prepared by a polypyrrole, a polyphenylenediamine, a glutaraldehyde-crosslinking or a photocrosslinking polymer PVA-SbQ, it was shown that the electro-conductivity is necessary for enzyme immobilization materials of the oxidase-HRP electrodes. When lactate oxidase or glutamate oxidase was immobilized with HRP in a polypyrrole, cathodic current similar to the GOD-HRP electrode was observed. Finally, by using a polyaniline membrane, the sensor response of the GOD-HRP electrode could be controlled by successive reduction and oxidation of a polyaniline membrane.
- 電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society
電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society 119(11), 593-597, 1999-11
The Institute of Electrical Engineers of Japan