RAPD-PCRおよびアイソザイムパターンによるギンブナー腹子のクローン性の証明 Clonal Nature of Offspings of Ginbuna Carassius langsdorfii by RAPD-PCR and Isozyme Patterns

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著者

    • 董 仕 DONG Shi
    • 高知大学農学部栽培漁業学科 Department of Aquaculture, Faculty of Agriculture, Kochi University

抄録

The eggs of Ginbuna <i>Carassius langsdorfii</i> (all female triploids) were inseminated by the sperm of a Gengoroubuna <i>Carassius cuvieri</i> and a commom carp <i>Cyprinus carpio</i> respectively. DNA extracted from blood of parents and offsprings were amplified using 20 random primers and separated by electrophoresis (RAPD-PCR). Isozymes of muscle and liver of parents and offsprings were assayed by the horizontal starch gel electrophoresis. Distinct differences by maternal fish were observed in the patterns of both RAPD-PCR (17 primers) and isozymes (CK, EST, GPI, PGM), but those of offsprings produced by crossing with Gengoroubuna and common carp respectively were the same completely within the maternal fish. Therefore, the results obtained in this study verified that the offsprings from Ginbuna were clonal genetically, being the same with maternal fish throughout the genome.

収録刊行物

  • 日本水産学会誌  

    日本水産学会誌 62(6), 891-896, 1996-11-15 

    日本水産學會

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各種コード

  • NII論文ID(NAID)
    10004850139
  • NII書誌ID(NCID)
    AN00193422
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00215392
  • NDL 記事登録ID
    4078368
  • NDL 雑誌分類
    ZR26(科学技術--農林水産--水産)
  • NDL 請求記号
    Z18-345
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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