Clonal Nature of Offspings of Ginbuna Carassius langsdorfii by RAPD-PCR and Isozyme Patterns.

• Dong Shi

  Department of Aquaculture, Faculty of Agriculture, Kochi University

• Taniguchi Nobuhiko

  Department of Aquaculture, Faculty of Agriculture, Kochi University

Bibliographic Information

Other Title

• ＲＡＰＤ‐ＰＣＲおよびアイソザイムパターンによるギンブナー腹子のクローン性の証明
• RAPD-PCRおよびアイソザイムパターンによるギンブナ一腹子のクローン性の証明
• RAPD-PCR オヨビ アイソザイム パターン ニヨル ギンブナ イチ ハラ

Abstract

The eggs of Ginbuna Carassius langsdorfii (all female triploids) were inseminated by the sperm of a Gengoroubuna Carassius cuvieri and a commom carp Cyprinus carpio respectively. DNA extracted from blood of parents and offsprings were amplified using 20 random primers and separated by electrophoresis (RAPD-PCR). Isozymes of muscle and liver of parents and offsprings were assayed by the horizontal starch gel electrophoresis. Distinct differences by maternal fish were observed in the patterns of both RAPD-PCR (17 primers) and isozymes (CK, EST, GPI, PGM), but those of offsprings produced by crossing with Gengoroubuna and common carp respectively were the same completely within the maternal fish. Therefore, the results obtained in this study verified that the offsprings from Ginbuna were clonal genetically, being the same with maternal fish throughout the genome.

Journal

• NIPPON SUISAN GAKKAISHI

  NIPPON SUISAN GAKKAISHI 62 (6), 891-896, 1996

  The Japanese Society of Fisheries Science

Keywords

• RAPD
• RAPD-PCR

Details

• CRID: 1390282681388654592
• NII Article ID: 10004850139
• NII Article ID: 10004850772
• NII Book ID: AN00193422
• DOI: 10.2331/suisan.62.891
• COI: 1:CAS:528:DyaK28Xns1ahurs%3D
• ISSN: 1349998X
• ISSN: 00215392
• NDL BIB ID: 4078368
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010552378
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4078368
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed