高水温処理によるギンブナ卵へのコイ精子の導入とDNAマーカーによる確認 Introduction of Sperm of Common Carp Cyprinus carpio into Eggs of Ginbuna Carassius Iangsdorfii by Heart Shock Treatment and Its Confirmation by DNA Markers
The eggs of a Ginbuna <i>Carassius langsdorfii</i> (all female triploids) were inseminated by the sperms of a common carp <i>Cyprinus carpio</i>. A part of the eggs was treated by heat shock: 40°C for 1.0min at 5min after fertilization.<br>The average of erythrocytic major diameter of offsprings from non-treated eggs was 17.4μm (17.1-17.7μm). The patterns of glucosephosphate isomerase (GPI), RAPD-PCR (random primer: OPA-04, 09, 15), and DNA fingerprinting (restriction endonuclease: <i>Hae</i> III; probe: YNZ 22) in the offsprings were identical with maternal fish.<br>The average of erythrocytic major diameter of offsprings from treated eggs was 20.6μm (20.3-20.9μm). The patterns of GPI, RAPD-PCR, and DNA fingerprinting in the offsprings were composed of all bands of maternal fish and partial bands of paternal fish.<br>Therefore, the results obtained in this study suggest that the offsprings from non-treated eggs were triploid clones, and the offsprings from treated eggs might be tetraploid composed of genome from both parents.
日本水産学会誌 63(2), 201-206, 1997-03-15