高水温処理によるギンブナ卵へのコイ精子の導入とDNAマーカーによる確認 Introduction of Sperm of Common Carp Cyprinus carpio into Eggs of Ginbuna Carassius Iangsdorfii by Heart Shock Treatment and Its Confirmation by DNA Markers

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The eggs of a Ginbuna <i>Carassius langsdorfii</i> (all female triploids) were inseminated by the sperms of a common carp <i>Cyprinus carpio</i>. A part of the eggs was treated by heat shock: 40°C for 1.0min at 5min after fertilization.<br>The average of erythrocytic major diameter of offsprings from non-treated eggs was 17.4μm (17.1-17.7μm). The patterns of glucosephosphate isomerase (GPI), RAPD-PCR (random primer: OPA-04, 09, 15), and DNA fingerprinting (restriction endonuclease: <i>Hae</i> III; probe: YNZ 22) in the offsprings were identical with maternal fish.<br>The average of erythrocytic major diameter of offsprings from treated eggs was 20.6μm (20.3-20.9μm). The patterns of GPI, RAPD-PCR, and DNA fingerprinting in the offsprings were composed of all bands of maternal fish and partial bands of paternal fish.<br>Therefore, the results obtained in this study suggest that the offsprings from non-treated eggs were triploid clones, and the offsprings from treated eggs might be tetraploid composed of genome from both parents.

収録刊行物

  • 日本水産学会誌  

    日本水産学会誌 63(2), 201-206, 1997-03-15 

    日本水産學會

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各種コード

  • NII論文ID(NAID)
    10004850767
  • NII書誌ID(NCID)
    AN00193422
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00215392
  • NDL 記事登録ID
    4173461
  • NDL 雑誌分類
    ZR26(科学技術--農林水産--水産)
  • NDL 請求記号
    Z18-345
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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