Introduction of Sperm of Common Carp Cyprinus carpio into Eggs of Ginbuna Carassius langsdorfii by Heat Shock Treatment and Its Confirmation by DNA Markers.

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  • Dong Shi
    Department of Aquaculture, Faculty of Agriculture, Kochi University
  • Ohara Kenichi
    Department of Aquaculture, Faculty of Agriculture, Kochi University
  • Taniguchi Nobuhiko
    Department of Aquaculture, Faculty of Agriculture, Kochi University

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  • 高水温処理によるギンブナ卵へのコイ精子の導入とDNAマーカーによる確認
  • コウ スイオン ショリ ニヨル ギンブナ ラン エ ノ コイ セイシ ノ ドウ

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Abstract

The eggs of a Ginbuna Carassius langsdorfii (all female triploids) were inseminated by the sperms of a common carp Cyprinus carpio. A part of the eggs was treated by heat shock: 40°C for 1.0min at 5min after fertilization.<br>The average of erythrocytic major diameter of offsprings from non-treated eggs was 17.4μm (17.1-17.7μm). The patterns of glucosephosphate isomerase (GPI), RAPD-PCR (random primer: OPA-04, 09, 15), and DNA fingerprinting (restriction endonuclease: Hae III; probe: YNZ 22) in the offsprings were identical with maternal fish.<br>The average of erythrocytic major diameter of offsprings from treated eggs was 20.6μm (20.3-20.9μm). The patterns of GPI, RAPD-PCR, and DNA fingerprinting in the offsprings were composed of all bands of maternal fish and partial bands of paternal fish.<br>Therefore, the results obtained in this study suggest that the offsprings from non-treated eggs were triploid clones, and the offsprings from treated eggs might be tetraploid composed of genome from both parents.

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