Na^+チャネル阻害物質の活性測定用センサに用いたカエル膀胱膜の貯蔵条件 Storage Conditions of the Frog Bladder Membrane on Using for the Na^+ Channel Blocker Sensor
A simple biosensor for measuring sodium channel blockers, such as tetrodotoxin (TTX) and saxitoxin (STX), and paralytic shellfish poisoning (PSP) consisted of frog bladder membrane, and Na<sup>+</sup> electrode. Effects of storage temperature, antiseptic and species of frog on the activity of Na<sup>+</sup> channel on the membrane were investigated from the standpoint of practical use.<br>The results obtained were as follows:<br>1) The maximum output of the sensor was obtained at 30 to 33°C and pH 4.8 to 6.0.<br>These values were shown higher in the case of tropical frog than a temperate frog. This was assumed due to the effect of living the environment.<br>2) Frog bladder was able to keep at 5°C during 7 months, but at -30°C during only 80 days.<br>3) Antiseptic effect of NaN<sub>3</sub> on the frog bladder membrane was recognized in the concentration of 0.003%.
日本水産学会誌 63(4), 616-620, 1997-07-15