アメマスSalvelinus leucomaenisビテロゲニンの卵黄蛋白質への特異的分解 Specific Proteolysis of Vitellogenin to Egg Yolk Proteins in White Spotted-charr Salvelinus leucomaenis

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著者

    • 平松 尚史 HIRAMATSU Naoshi
    • 北海道大学水産学部水産増殖学科 Laboratory of Fresh-Water Fish-Culture, Department of Biology and Aquaculture, Faculty of Fisheries, Hokkaido University
    • 原 彰彦 HARA Akihiko
    • 北海道大学水産学部附属七飯養魚実習施設 Nanae Fish Culture Experimental Station, Faculty of Fisheries, Hokkaido University

抄録

Vitellogenin (Vg) was purified from white spotted-charr <i>Salvelinus leucomaenis</i> serum and biotinylated (b-Vg). The b-Vg was digested under mild acidic conditions by bovine cathepsin-D (BCD) or a water soluble fraction of charr eggs (WSF), Digests were analyzed by 5.0-22.5% gradient SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Westem blotting, and gel filtration on Superose 6. Westem blotting was done using: 1) streptavidin coupled to horseradish peroxidase (HRPO) to identify biotinylated products of b-Vg digestion, and 2) antisera against lipovitellin (a-Lv) or β-component (a-β') to immunologically relate digestion products to specific charr egg yolk proteins. With respect to antigenicity and apparent molecular weight, fragments resulting from digestion of b-Vg by WSF or BCD were almost indistinguishable from those found in charr egg yolk, except for phosvitin (Pv). Pv-related phosphorus in BCD digests eluted at a position corresponding to a molecular weight slightly greater than that of Pv in egg yolk. The present study is the first to demonstrate <i>in vitro</i> conversion of salmonid Vg to its related egg yolk proteins. The similar catalytic effect of WSF or BCD on <i>in vitro</i> generation of egg yolk proteins suggests that cathepsin-D, present in WSF or intact eggs, may play a key role in natural yolk formation in salmonids.

収録刊行物

  • 日本水産学会誌  

    日本水産学会誌 63(5), 701-708, 1997-09-15 

    日本水産學會

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各種コード

  • NII論文ID(NAID)
    10004851786
  • NII書誌ID(NCID)
    AN00193422
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00215392
  • NDL 記事登録ID
    4305146
  • NDL 雑誌分類
    ZR26(科学技術--農林水産--水産)
  • NDL 請求記号
    Z18-345
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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