トラフグ養成親魚からの採卵技法の開発 [in Japanese] Development of the Maturation-and Ovulation-induction Method in Cultured Tiger Puffer Takifugu rubripes by Hormonal Treatments [in Japanese]
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We developed an effective method for the induction of oocyte maturation and ovulation in the cultured tiger puffer <i>Takifugu rubripes</i> by hormonal treatments, in order to supply the fertilized eggs for seedling production. Ovarian biopsy just prior to the hormonal treatments revealed the oocyte diameter and developmental stages of the oocytes.<br>A luteinizing hormone-releasing hormone analogue (LHRH-a) cholesterol pellet (400μg/kg) implantation successfully induced oocyte maturation for three-year-old cultured tiger puffer which had yolk accumulated ovaries. Thereafter, single injection of HCG (500IU/kg) combined with chum salmon pituitary (SP) homogenate (7mg/kg) was applied to the fish to synchronize ovulation among fish. Ovulation mainly occurred between 96 and 120 hours after HCG+SP injection. Thus, a technique for obtaining eggs of high quality from the cultured tiger puffer was developed.
NSUGAF 63(5), 728-733, 1997-09-15
The Japanese Society of Fisheries Science