Regulation of Carbohydrate Metabolism in Fish-XXXI. Concentrations of Metabolic Intermediates and Their Relation to Phosphofructokinase Activity in Carp Hepatopancreas.

• Shimeno Sadao

  Laboratory of Fish Nutrition, Faculty of Agriculture, Kochi University

• Shikata Takafumi

  Laboratory of Fish Nutrition, Faculty of Agriculture, Kochi University Ishikawa Prefecture Fisheries Research Center

• Iwanaga Shunsuke

  Laboratory of Fish Nutrition, Faculty of Agriculture, Kochi University Uwajima City Fisheries Seeding Production Center

• Sugita Tsuyoshi

  Laboratory of Fish Nutrition, Faculty of Agriculture, Kochi University

Bibliographic Information

Other Title

• 魚類糖代謝の調節　ＸＸＸＩ　　コイ肝すい臓における代謝中間体の含量およびｐｈｏｓｐｈｏｆｒｕｃｔｏｋｉｎａｓｅ活性への関与
• コイ肝膵臓における代謝中間体の含量およびphosphofructokinase活性への関与
• コイ カン スイゾウ ニ オケル タイシャ チュウカンタイ ノ ガンリョウ オ

Abstract

The metabolic intermediate concentrations and enzyme activities were determined in the hepatopancreas of carp Cyprinus carpio fed for 30 days with diets high in protein, lipid, and carbohy-drate. The effect of physiological concentrations of the intermediates on the activity of purified phos-phofructokinase (PFK) was also investigated. The dietary treatments induced slow but marked changes in activities of hepatopancreatic enzymes. This suggested an enzyme induction in the fish hepatopancreas but could not account for the rapid response of body composition to the dietary treat-ments. Each concentration of ATP, ADP, AMP, G1P, G6P, F6P, and FDP in the hepatopancreas ranged from 10^-3M to 10^-5M, corresponding to the Km values for many glycolytic enzymes such as PFK. These physiological concentration ranges of ATP, AMP, F6P, and citrate markedly and reasonably affected in vitro PFK activity. The results suggest that in vivo glycolytic activity can be regulated not only by enzyme induction but also by PFK activity without enzyme induction through changes in the physiological concentrations of metabolic intermediates as substrate and effector.

Journal

• NIPPON SUISAN GAKKAISHI

  NIPPON SUISAN GAKKAISHI 64 (1), 110-115, 1998

  The Japanese Society of Fisheries Science

Keywords

• phosphofructokinase

Details

• CRID: 1390001206411499136
• NII Article ID: 10004852651
• NII Book ID: AN00193422
• DOI: 10.2331/suisan.64.110
• ISSN: 1349998X
• ISSN: 00215392
• NDL BIB ID: 4381368
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030571928
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4381368
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed