ニチリンヒトデ幽門盲のうホスホリパーゼA_2様酵素の精製と性質 Purification and Properties of Phospholipase A_2-like Enzyme from the Pyloric Caeca of the Starfish Solaster Paxillatus

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Phospholipase A<sub>2</sub>-like enzyme was purified from the crude extracts of the pyloric caeca of the starfish <i>Solaster paxillatus</i> by heat treatment followed by gel filtration on a Sephacryl S-200, preparative polyacrylamide gel electrophoresis, and gel filtration on a Sephadex G-50.<br>The final phospholipase A<sub>2</sub>-1ike enzyme preparation was nearly homogeneous in SDS-polyacrylamide gel electrophoresis, and its molecular weight was estimated to be approximately 13, 000. The enzyme mainly released oleic acid from 1-palmitoyl-2-o1eoy1-<i>sn</i>-glycero-3-phosphocholine. For hydrolysis of egg yolk phosphatidylcho1ine, the optimum pH and temperature of this enzyme were in the range of pH 9-10 and 30-40°C, respectively, and the enzyme was activated by Ca<sup>2+</sup>. Starfish phospholipase A<sub>2</sub>-1ike enzyme as well as porcine pancreatic phospholipase A<sub>2</sub> did not show the fatty acid specificity for hydrolysis of phosphatidylcholine prepared from scallop adductor muscle.

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  • 日本水産学会誌  

    日本水産学会誌 64(2), 264-269, 1998-03-15 

    日本水産學會

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各種コード

  • NII論文ID(NAID)
    10004853033
  • NII書誌ID(NCID)
    AN00193422
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00215392
  • NDL 記事登録ID
    4437717
  • NDL 雑誌分類
    ZR26(科学技術--農林水産--水産)
  • NDL 請求記号
    Z18-345
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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