ニチリンヒトデ幽門盲のうホスホリパーゼA2様酵素の精製と性質
書誌事項
- タイトル別名
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- Purification and Properties of Phospholipase A2-like Enzyme from the Pyloric Caeca of the Starfish Solaster paxillatus.
- ニチリンヒトデ ユウモン モウノウ ホスホリパーゼ A2ヨウ コウソ ノ セイ
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抄録
Phospholipase A2-like enzyme was purified from the crude extracts of the pyloric caeca of the starfish Solaster paxillatus by heat treatment followed by gel filtration on a Sephacryl S-200, preparative polyacrylamide gel electrophoresis, and gel filtration on a Sephadex G-50.<br>The final phospholipase A2-1ike enzyme preparation was nearly homogeneous in SDS-polyacrylamide gel electrophoresis, and its molecular weight was estimated to be approximately 13, 000. The enzyme mainly released oleic acid from 1-palmitoyl-2-o1eoy1-sn-glycero-3-phosphocholine. For hydrolysis of egg yolk phosphatidylcho1ine, the optimum pH and temperature of this enzyme were in the range of pH 9-10 and 30-40°C, respectively, and the enzyme was activated by Ca2+. Starfish phospholipase A2-1ike enzyme as well as porcine pancreatic phospholipase A2 did not show the fatty acid specificity for hydrolysis of phosphatidylcholine prepared from scallop adductor muscle.
収録刊行物
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- 日本水産学会誌
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日本水産学会誌 64 (2), 264-269, 1998
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390282681387224064
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- NII論文ID
- 10004856472
- 10004853033
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- NII書誌ID
- AN00193422
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- COI
- 1:CAS:528:DyaK1cXjsFeks70%3D
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- ISSN
- 1349998X
- 00215392
- http://id.crossref.org/issn/00215392
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- NDL書誌ID
- 4437717
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可