ニチリンヒトデ幽門盲のうホスホリパーゼＡ２様酵素の精製と性質

• 岸村 栄毅

  北海道大学水産学部

• 林 賢治

  北海道大学水産学部

書誌事項

タイトル別名

• Purification and Properties of Phospholipase A2-like Enzyme from the Pyloric Caeca of the Starfish Solaster paxillatus.
• ニチリンヒトデ ユウモン モウノウ ホスホリパーゼ A2ヨウ コウソ ノ セイ

抄録

Phospholipase A₂-like enzyme was purified from the crude extracts of the pyloric caeca of the starfish Solaster paxillatus by heat treatment followed by gel filtration on a Sephacryl S-200, preparative polyacrylamide gel electrophoresis, and gel filtration on a Sephadex G-50.<br>The final phospholipase A₂-1ike enzyme preparation was nearly homogeneous in SDS-polyacrylamide gel electrophoresis, and its molecular weight was estimated to be approximately 13, 000. The enzyme mainly released oleic acid from 1-palmitoyl-2-o1eoy1-sn-glycero-3-phosphocholine. For hydrolysis of egg yolk phosphatidylcho1ine, the optimum pH and temperature of this enzyme were in the range of pH 9-10 and 30-40°C, respectively, and the enzyme was activated by Ca²⁺. Starfish phospholipase A₂-1ike enzyme as well as porcine pancreatic phospholipase A₂ did not show the fatty acid specificity for hydrolysis of phosphatidylcholine prepared from scallop adductor muscle.

収録刊行物

• 日本水産学会誌

  日本水産学会誌 64 (2), 264-269, 1998

  公益社団法人 日本水産学会

キーワード

• ホスホリパーゼA₂様酵素
• 幽門盲のう
• ニチリンヒトデ

詳細情報

• CRID: 1390282681387224064
• NII論文ID: 10004856472
• NII論文ID: 10004853033
• NII書誌ID: AN00193422
• DOI: 10.2331/suisan.64.264
• COI: 1:CAS:528:DyaK1cXjsFeks70%3D
• ISSN: 1349998X
• ISSN: 00215392
• ISSN: http://id.crossref.org/issn/00215392
• NDL書誌ID: 4437717
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030572385
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4437717
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可