ミトコンドリアDNAの制限酵素切断型分析によるヒラメ天然集団の遺伝的変異 Genetic variation in the Japanese Flounder Based on Analysis of Mitochondrial DNA by Restriction Endonucleases
Mitochondrial DNA (mtDNA) of the Japanese flounder collected from eight localities around Japan were examined to clarify the genetic feature and population structure. Digestion of region encompassing from 3' half of cytochrome <i>b</i> to the left and central domain of D-loop containing region of the flounder mtDNA amplified by Polymerase Chain Reaction (PCR) by eleven restriction endonucleases revealed considerable variation. We determined 54 cleavage sites by eleven enzymes and found 38 haplotypes in 75 specimens. Restriction patterns were polymorphic for ten enzymes examined. Nucleotide divergence between haplotypes ranged from 0.24 to 2.69%. Dendrogram constructed by the UPGMA among 38 haplotypes showed no clustering groups of localities. Nucleotide diversity and haplotypic diversity of each locality ranged from 0.0031 to 0.0042 and from 0.82 to 0.93, respectively. These results showed the Japanese flounder have a high mtDNA variability. The results of the present study provide some basic information for population genetics, and are useful for development of the genetic monitoring method for stocking of hatchery-raised fish fry into natural waters.
日本水産学会誌 64(3), 377-383, 1998-05-15