Amino acid sequence of phospholipase A_2 from the pyloric ceca of starfish Asterina pectinifera

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The complete amino acid sequence of phospholipase A<sub>2</sub> (PLA<sub>2</sub>) from the pyloric ceca of the starfish <i>Asterina pectinifera</i> was determined by automated Edman degradation. The <i>A</i>. <i>pectinifera</i> PLA<sub>2</sub> (APLA<sub>2</sub>) consists of 137 amino acids with an unblocked N-terminus and its molecular weight is calculated to be 15 300.1. The enzyme contains 14 cysteine (Cys) residues at the corresponding positions of the same residues which have been shown to be involved in intramolecular disulfide bonds in mammalian pancreatic PLA<sub>2</sub>. The region involving an active site and a Ca<sup>2+</sup>-binding loop shows fairly high sequence homology (75%) between the APLA<sub>2</sub> and porcine pancreatic PLA<sub>2</sub>. The APLA<sub>2</sub> conserved the amino acid sequence of the loop portion of the porcine pancreatic PLA<sub>2</sub> except for the deletion of two amino acids. These features indicate that the APLA<sub>2</sub> can be classified into the group 1 type PLA<sub>2</sub>. In contrast, the homology between the APLA<sub>2</sub> and porcine pancreatic PLA<sub>2</sub> was calculated to be 47% in the whole region. Further, the insertion of sixteen residues and the deletion of three residues were required in the sequence of the APLA<sub>2</sub> to align the corresponding region to the β-wing of porcine pancreatic PLA<sub>2</sub>. These differences in amino acid sequence of the APLA<sub>2</sub> may account for its specific properties such as the higher activity and the characteristic substrate specificity.

収録刊行物

  • Fisheries science : FS  

    Fisheries science : FS 66(1), 104-109, 2000 

    The Japanese Society of Fisheries Science

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各種コード

  • NII論文ID(NAID)
    10004857347
  • NII書誌ID(NCID)
    AA10993718
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09199268
  • NDL 記事登録ID
    5296929
  • NDL 雑誌分類
    ZR26(科学技術--農林水産--水産)
  • NDL 請求記号
    Z54-H592
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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