Production of Anti-Infectious Hematopoietic Necrosis Virus (IHNV) Substances by Immobilized Whole Living Cells of Aquatic Bacteria in a Bioreactor System

• Myouga Hisashi

  Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University

• Yoshimizu Mamoru

  Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University

• Yumoto Isao

  Hokkaido National Industrial Research Institute

• Ezura Yoshio

  Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University

• Kimura Takahisa

  Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University

Bibliographic Information

Other Title

• Production of Anti Infectious Hematopoi

Abstract

A simple, low-cost and efficient bioreactor system for production of anti-infectious hematopoietic necrosis virus (IHNV) substances by bacteria isolated from aquatic environments was devised on a laboratory scale. Whole living cells of anti-IHNV substances producing bacteria, Pseudomonas sp. 51 BBW-29 and Alteromonas sp. 48 HS-27, were immobilized in calcium alginate beads prepared by 1% sodium alginate, 0.45M CaCl₂, and C₂Y or MC₂Y broth. Eighteen g of the beads were packed into a 25 ml reaction column and incubated at 15°C for 5 days in a circulating broth medium at a flow rate of 0.35 ml/h. After activation of the immobilized bacteria, the beads were incubated in a continuous flow of fresh broth with intermittent exposure to air. In the beads incubated under these conditions, the immobilized bacteria grew well at the gel surface and high viability of approximately 10¹⁰ CFU/bead was retained for at least 6 days from day 8 to 14. During 31 days of the experiment with the bioreactor system, the highest production was 69.3 ΔIHD₅₀ for Pseudomonas sp. 51 BBW-29 (at 14-17 days incubation) and 48.0 ΔIHD₅₀ for Alteromonas sp. 48 HS-27 (at 8-11 days incubation), which were 2.5 and 2.7 times higher than those of conventional batch culture (27.3 and 18.0 ΔIHD₅₀ for the two strains at 2-5 days incubation). The immobilized bacteria continuously produced high levels of anti-IHNV substances for 15 days (day 5-20).

Journal

• Fisheries science

  Fisheries science 61 (1), 61-64, 1995

  The Japanese Society of Fisheries Science

Keywords

• antiviral substance
• alginate beads
• bioreactor
• IHNV
• Pseudomonas
• Alteromonas

Details

• CRID: 1390282679404995456
• NII Article ID: 130003741972
• NII Article ID: 10004860736
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.61.61
• HANDLE: 2115/38579
• NDL BIB ID: 3644467
• ISSN: 09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3644467
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/61/1/61_1_61/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed