Cationic Constituents, Protein Concentration, and Osmolality of Coelomic Fluid in Salmonid Fish

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  • Cationic Constituents Protein Concentra

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Concentrations of four cations, protein content and osmolarity were measured on coelomic fluids and sera from 8 salmonid species. Sodium was the major cation inboth serum and coelomic fluid. The concentrations ranged 144-163mM in serum and 146-163mM in coelomic fluid and showed similar levels between serum and coelomic fluid in the same species. Meanwhile, potassium concentrations of both fluids varied in species (serum, 0.25-2.50mM; coelomic fluid 2.44-3.81mM), andtend tobe high in coelomic fluid. On the contrary, magnesium concentrations of coelomic fluid werelower than those of serum. Protein concentration of coelomic fluid wasabout 7-20% of that in serum in the individual species. Osmolality of coelomic fluid measured by the freezing point depression was only slightly but lower than the level of serum in all species. Immunoelectrophoretic test using antiserum against male serum proteins demonstrated that a part of coelomic fluid proteins originated from blood.

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