Occurrence of Multiple Lectins in the Hemolymph of Kuruma Prawn <i>Penaeus japonicus</i>
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- Muramoto Koji
- School of Fisheries Sciences, Kitasato University Faculty of Agriculture, Tohoku University
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- Matsuda Takuya
- School of Fisheries Sciences, Kitasato University
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- Nakada Keisuke
- School of Fisheries Sciences, Kitasato University
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- Kamiya Hisao
- School of Fisheries Sciences, Kitasato University
Bibliographic Information
- Other Title
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- Occurrence of Multiple Lectins in the Hemolymph of Kuruma Prawn Penaeus japonicus
- Occurrence of Multiple Lectins in the H
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Abstract
The hemolymph of kuruma prawn Penaeus japonicus agglutinated horse, sheep, chicken, and human erythrocytes, but not a marine bacterium Vibrio anguillarum. The hemagglutinating activity was dependent on the presence of calcium ions. The higher titer values were obtained by keeping the reaction mixture at 6°C for 16 h compared to those observed at room temperature. The hemagglutinating activity was inhibited by glycoproteins and simple sugars such as mucin Type I, mucin Type II, fetuin, D-mannosamine, N-acetyl-D-glucosamine, and N-acetylneuraminic acid. The lectins were purified by affinity chromatography on mucin Type II-Sepharose 6 B. Succeeding separation by ion-exchange chromatography and gel-filtration revealed that the affinity purified lectins were a mixture of the lectins with different molecular species and also sugar-binding specificity. The two lectins were obtained in electrophoretically pure form; one (Fraction B-1) was a protein of 370 kDa consisted of subunits of 84 kDa and the other (Fraction D, 240 kDa) consisted with subunits of 34 kDa.
Journal
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- Fisheries science
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Fisheries science 61 (1), 131-135, 1995
The Japanese Society of Fisheries Science
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Details
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- CRID
- 1390282679404986112
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- NII Article ID
- 130003741981
- 10004861020
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- NII Book ID
- AA10993718
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- NDL BIB ID
- 3644320
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- ISSN
- 09199268
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed