Development of a Sensitive Sandwich Enzyme-Linked Immunosorbent Assay(ELISA) for Vitellogenin in the Japanese Eel Anguilla japonica

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  • Okumura Hiromi
    Department of Biology, Facutly of Fisheries, Hokkaido University
  • Hara Akihiko
    Nanae Fish Culture Experimental Station, Facutlty of Fisheries, Hokkaido University
  • Saeki Fumihiro
    Department of Biology, Facutly of Fisheries, Hokkaido University
  • Todo Takashi
    Department of Biology, Facutly of Fisheries, Hokkaido University
  • Adachi Shinji
    Department of Biology, Facutly of Fisheries, Hokkaido University
  • Yamauchi Kohei
    Department of Biology, Facutly of Fisheries, Hokkaido University

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タイトル別名
  • Development of a Sensitive Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) for Vitellogenin in the Japanese Eel <i>Anguilla japonica</i>
  • Development of a Sensitive Sandwich Enz

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A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of low levels of serum vitellogenin (VTG) of the Japanese eel Anguilla japonica. The non-competitive avidin-biotm interaction was performed by a sandwich method using the specificity of the specific antiserum to eel yolk protein (a-lipovitellin) and biotinylated F (ab')2 fragment of a-lipovitellin. The assay could be run in 2 days and routinely detect VTG in a concentration as low as 0.8 ng/ml. The appearance of low levels of serum VTG significantly increased 9-12h after a single injection of estradiol-17β into immature fish.The development of an ELISA for VTG made possible the quantification of serum VTG and thereby in vitro analysis of the mechanism of the onset and course of vitellogenesis.

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