Partial Characterization of a Calcitonin-like Substance from the Brockmann Bodies of the Medaka Oryzias latipes

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  • Partial Characterization of a Calcitonin-like Substance from the Brockmann Bodies of the Medaka <i>Oryzias latipes</i>
  • Partial Characterization of a Calcitoni

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Biochemical natures of calcitonin (CT)-like substance obtained from Brockmann bodies (BB) ofthe medaka Oryzias latipes (Teleostei) were examined. A crude extract of BB was fractionated byreversed-phase HPLC and screened with anti-salmon CT antisetum by Western blotting. Although 4 fractions showed positive immunoreactivity, CT-like biological activity was detected only in one ofthese fractions. The molecular weight of the identified CT-like substance was calculated as 20 kDa, which is considerably higher than that (3.5 kDa) of authentic CTs of other vertebrates.

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