Development of an Enzyme Sensor Using Multi-enzyme System Immobilized in β-Chitin Membrane
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- Ohashi Eiji
- Central Research Laboratory, Nippon Suisan Kaisha, Ltd.
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- Karube Isao
- Research Center for Advanced Science and Technology, University of Tokyo
Bibliographic Information
- Other Title
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- Development of an Enzyme Sensor Using M
- Development of an Enzyme Sensor Using Multi-enzyme System Immobilized in β-Chitin Membrane
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Abstract
A multi-enzyme system was immobilized in a β-chitin membrane. β-Chitin was obtained from common squid pens and glutamate pyruvate transaminase and pyruvate oxidase were immobilized simultaneously in the β-chitin membrane. The immobilization was achieved by filtration of the chitin suspension containing enzymes without any chemical reagent or pH treatment. An enzyme electrode for the measurement of L-alanine was assembled with the β-chitin membrane and an oxygenelectrode, and enzyme activities were evaluated. A control membrane was prepared using a cross-linking method with enzymes, bovine serum albumin and glutaraldehyde. The electrode response of the β-chitin membrane was obtained immediately after the sample injection and leveled off in 60 s. In contrast, the control membrane showed a slower response. The maximum response of the β-chitin embrane electrode was obtained at 38°C and pH 7.1. Linearity of the calibration curve was obtained up to 30mM in the batch system and 200mM in the flow system. These results suggest that β-chitin is a useful supporting material for enzyme immobilization and applicable to enzyme electrodes.
Journal
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- Fisheries science
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Fisheries science 61 (5), 856-859, 1995
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390001204430354560
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- NII Article ID
- 130003902795
- 10004863797
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- NII Book ID
- AA10993718
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- NDL BIB ID
- 3642540
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- ISSN
- 09199268
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed