Identification of Clones Induced by Chromosome Manipulation in Ayu <i>Plecoglossus altivelis</i> by DNA Fingerprinting with RI and Non-RI Labelled Probes
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- Takagi Motohiro
- Department of Cultural Fisheries, Faculty of Agriculture, Kochi University
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- Taniguchi Nobuhiko
- Department of Cultural Fisheries, Faculty of Agriculture, Kochi University
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- Yamasaki Motoshi
- Department of Cultural Fisheries, Faculty of Agriculture, Kochi University
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- Tsujimura Akio
- Freshwater Fisheries Center of Wakayama Prefecture
Bibliographic Information
- Other Title
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- Identification of Clones Induced by Chromosome Manipulation in Ayu Plecoglossus altivelis by DNA Fingerprinting with RI and Non-RI Labelled Probes
- Identification of Clones Induced by Chr
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Abstract
DNA fingerprint (DNA-FP) was applied for confirmation and identification of clones induced by the suppression of the first cleavage in ayu, Plecoglossus altivelis. The samples used were 2 homozygous clonal lines, 3 successive homozygous clonal lines and 2 heterozygous clonal lines. We examined suitable enzyme/probe combinations and compared radioisotope (RI) and non-radioisotope (Non-RI) labelled probes to get clear DNA-FP.<br> The clear DNA fingerprints were obtained by using YNZ 22, 33. 15 and 33.6 probes with Hae III, Msp I and Hinf I restriction enzymes. In these DNA-FPs, the bands were separated distinctly throughout the membrane in both higher and lower molecular weight regions. The DNA-FP bands obtained with the Non-RI labelled 33.15 probes were as intense and clearly separated as those obtained with the RI method. All the fragments were shared among individuals within the same clonal line, but the pattern of bands detected was significantly different between clonal lines.<br> It was confirmed that DNA-FP is effective for the identification of clonal lines. DNA-FP pattern by Non-RI labelled probe would be suitable for wide applications in studies on inbreeding and identification of individuals and families.
Journal
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- Fisheries science
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Fisheries science 61 (6), 909-914, 1995
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390282679407108992
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- NII Article ID
- 130003902801
- 10004864028
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- NII Book ID
- AA10993718
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- NDL BIB ID
- 3920557
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- ISSN
- 09199268
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed