Sequences of cDNA Clones Encoding α-Actin of Carp and Goldfish Skeletal Muscles
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- Watabe Shugo
- Laboratory of Marine Biochemistry, Faculty of Agriculture, The University of Tokyo
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- Hirayama Yasushi
- Laboratory of Marine Biochemistry, Faculty of Agriculture, The University of Tokyo
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- Imai Jun-ichi
- Laboratory of Marine Biochemistry, Faculty of Agriculture, The University of Tokyo
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- Kikuchi Kiyoshi
- Laboratory of Fish Physiology, Faculty of Agrculture, The University of Tokyo
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- Yamashita Michiaki
- National Research Institute of Fisheries Science
Bibliographic Information
- Other Title
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- Sequences of c DNA Clones Encoding アルファ
- Sequences of cDNA Clones Encoding α-Actin of Carp and Goldfish Skeletal Muscles
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Abstract
We have isolated cDNA clones encoding skeletal muscle α-actins of carp Cyprinus carpio and goldfish Carassius auratus. The complete nucleotide (nt) sequences ofcDNA clones and their deduced amino acid sequences were determined. The coding region of cDNA clones contained 1131 nt for both carp and goldfish, while their 3' untraslated regions showed polyadenylation signals. When the two cDNA nucleotide sequences were compared in the overlapping 1257 nt corresponding to the coordinate from -36 to 1221, 35 nt substitutions were observed. Among these, 21 substitutions occurred between T and C, while 25 of total 28 nt substitutions in the coding region were located at the third base. Totally 377 amino acids were deduced from the coding region of both carp and goldfish, although it was predicted that the two N-terminal amino acid residues, Met followed by Cys, would be processed after translation as the cases of other α-actins so far reported. Amino acid sequence differences between carp and goldfish mature α-actins were Asp/Glu-3 and Thr/Val-5.
Journal
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- Fisheries science
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Fisheries science 61 (6), 998-1003, 1995
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390001204428860032
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- NII Article ID
- 130003902832
- 10004864342
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- NII Book ID
- AA10993718
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- COI
- 1:CAS:528:DyaK28XhvVKmsA%3D%3D
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- NDL BIB ID
- 3920576
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- ISSN
- 09199268
- http://id.crossref.org/issn/09199268
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed