Molecular Weight Determination of Agar by Sedimentation Equilibrium Measurements
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- Tashiro Yuri
- Department of Foods Science and Technology, Tokyo University of Fisheries
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- Mochizuki Yoshinori
- Department of Foods Science and Technology, Tokyo University of Fisheries
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- Ogawa Hiroo
- Department of Foods Science and Technology, Tokyo University of Fisheries
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- Mizuno Haruo
- Department of Foods Science and Technology, Tokyo University of Fisheries
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- Iso Naomichi
- Department of Foods Science and Technology, Tokyo University of Fisheries
Bibliographic Information
- Other Title
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- Molecular Weight Determination of Agar
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Abstract
Sedimentation equilibrium and viscosity measurements were carried out on six agar samples in 0.1M KCl at 65°C. The sedimentation equilibrium data showed that the weight-average molecular weight, Mw, of these agar samples ranged from 3.0×104 to 1.9×105. The ratio of z-average molecular weight, Mz, to Mw, , which indicated the inhomogeneity of molecular weight, ranged from 1.1 to 2.9. The wide moleculare weight distribution suggested the cleavage of agar molecules during alkali treatment. Although the gelation point depended on molecular weight in the case of the same origin sample, the molecular weight dependence of gelation point did not exist in different algal species. From the log-log plots of intrinsic viscosity, [η], versus _??_w, it was clear that the data plots approximated the following equation: [η]=8.75×10-4 M0.68. From the magnitude of the exponent value, 0.68, we considered that agar molecules were in the form of random coils in0.1M KCl at 65°C. Furthermore, the molecular shape of agar in the case of adding an inhibitor of hydrogen bonding to the solution was also random coils, which supported the view that the sol-gel transition of agar is due to the formation of hydrogen bonds.
Journal
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- Fisheries science
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Fisheries science 62 (1), 80-83, 1996
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390282679406191232
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- NII Article ID
- 130003902846
- 10004864904
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- NII Book ID
- AA10993718
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- NDL BIB ID
- 3929049
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- ISSN
- 09199268
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed