Purification and Characterization of Alcohol Dehydrogenase from Liver of Skipjack Katsuwonus pelamis
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- Nagai Takeshi
- Department of Food Science and Technology, National Fisheries University
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- Hamada Moritsugu
- Department of Food Science and Technology, National Fisheries University
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- Kai Norihisa
- Department of Food Science and Technology, National Fisheries University
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- Tanoue Yasuhiro
- Department of Food Science and Technology, National Fisheries University
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- Nagayama Fumio
- Department of Food Science and Technology, Tokyo University of Fisheries School of Marine Science and Technology, Tokai University Akita Junior College
書誌事項
- タイトル別名
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- Purification and Characterization of Alcohol Dehydrogenase from Liver of Skipjack <i>Katsuwonus pelamis</i>
- Purification and Characterization of Al
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Alcohol dehydrogenase (EC 1. 1. 1. 1) in skipjack liver was extracted with sodium phosphate buffer solution and purified by ammonium sulfate fractionation and chromatographies on Toyopearl HW-55F, Butyl-Toyopearl 650M, and Blue-Toyopearl 650 ML. At the final step, it was separated into two fractions (ADH-1 and ADH-2). By this method, a final specific activity (ADH-1) of 590 units/mg and purification of 118.0-fold was attained. With respect to ADH-2, a final specificactivity of 1063 units/mg and purification of 212.6-fold was achieved. The apparent molecular weights were estimated to be about 140, 000 (ADH-1) and 130, 000 (ADH-2) by gel filtration on Toyopearl HW-55F. ADH-1 gave a single protein band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis which also revealed that this enzyme was composed of identical subunits with a molecular weight of 33, 000. ADH-2 showed a single band with MW of 66, 000. In relation to ADH-2, the optimum temperature was about 40°C. ADH-2 was stable at 30°C for 30min but completely inactivated at 40°C for 30min. The optimum pH was about 10 and ADH-2 was stable at pH 7-9 but was unstable when the pH was lower than 7.0. ADH-2 was activated by Co2+ and Mn2+, but was inhibited by Hg2+, Zn2+, and Cu2+.
収録刊行物
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- Fisheries science
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Fisheries science 62 (2), 272-277, 1996
公益社団法人 日本水産学会
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詳細情報
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- CRID
- 1390001204427959296
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- NII論文ID
- 130003902899
- 10004865646
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- NII書誌ID
- AA10993718
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- COI
- 1:CAS:528:DyaK28XisFGnsL4%3D
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- NDL書誌ID
- 3951203
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- ISSN
- 09199268
- http://id.crossref.org/issn/09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可