Production of Androgenetic Diploids in Amago Salmon Oncorhynchus masou ishikawae

• Nagoya Hiroyuki

  Inland Station, National Research Institute of Aquaculture

• Okamoto Hiroyuki

  Inland Station, National Research Institute of Aquaculture

• Nakayama Ichiro

  Inland Station, National Research Institute of Aquaculture

• Araki Kazuo

  Inland Station, National Research Institute of Aquaculture

• Onozato Hiroshi

  Department of Biology, Faculty of Science, Shinshu University

書誌事項

タイトル別名

• Production of Androgenetic Diploids in Amago Salmon <i>Oncorhynchus masou ishikawae</i>
• Production of Androgenetic Diploids in

抄録

The optimal conditions to induce androgenetic diploids using ⁶⁰Co gamma irradiation and hydrostatic pressure shock in amago salmon Oncorhynchus masou ishikawae were determined. Androgenetic haploids were produced when eggs were irradiated prior to fertilization, with an optimal dose of 450 Gray. Diploid amago salmon were produced from irradiated eggs by suppression of the first cleavage after fertilization with normal sperm. The optimal timing of the hydrostatic pressure shock (650 kgf/cm², 6min) was determined to be 7.5 hours after insemination at 10°C. DNA fingerprinting showed that the androgenetic offspring (second generation in androgenesis) were clones, thereby demonstrating that the androgenetic diploids (first generation in androgenesis) produced in these experiments were completely homozygous.

収録刊行物

• Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ

  Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ 62 (3), 380-383, 1996

  公益社団法人 日本水産学会

キーワード

• androgenesis
• homozygote
• amago salmon
• Oncorhynchus masou ishikawae

詳細情報

• CRID: 1390001204430056832
• NII論文ID: 130003741990
• NII論文ID: 10004866021
• NII書誌ID: AA10993718
• DOI: 10.2331/fishsci.62.380
• COI: 1:CAS:528:DyaK28XjvFyrtLo%3D
• NDL書誌ID: 3978832
• ISSN: 09199268
• ISSN: http://id.crossref.org/issn/09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3978832
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/62/3/62_3_380/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可