Production of Androgenetic Diploids in Amago Salmon <i>Oncorhynchus masou ishikawae</i>

• Nagoya Hiroyuki

  Inland Station, National Research Institute of Aquaculture

• Okamoto Hiroyuki

  Inland Station, National Research Institute of Aquaculture

• Nakayama Ichiro

  Inland Station, National Research Institute of Aquaculture

• Araki Kazuo

  Inland Station, National Research Institute of Aquaculture

• Onozato Hiroshi

  Department of Biology, Faculty of Science, Shinshu University

Bibliographic Information

Other Title

• Production of Androgenetic Diploids in Amago Salmon Oncorhynchus masou ishikawae
• Production of Androgenetic Diploids in

Abstract

The optimal conditions to induce androgenetic diploids using ⁶⁰Co gamma irradiation and hydrostatic pressure shock in amago salmon Oncorhynchus masou ishikawae were determined. Androgenetic haploids were produced when eggs were irradiated prior to fertilization, with an optimal dose of 450 Gray. Diploid amago salmon were produced from irradiated eggs by suppression of the first cleavage after fertilization with normal sperm. The optimal timing of the hydrostatic pressure shock (650 kgf/cm², 6min) was determined to be 7.5 hours after insemination at 10°C. DNA fingerprinting showed that the androgenetic offspring (second generation in androgenesis) were clones, thereby demonstrating that the androgenetic diploids (first generation in androgenesis) produced in these experiments were completely homozygous.

Journal

• Fisheries science

  Fisheries science 62 (3), 380-383, 1996

  The Japanese Society of Fisheries Science

Keywords

• androgenesis
• homozygote
• amago salmon
• Oncorhynchus masou ishikawae

Details

• CRID: 1390001204430056832
• NII Article ID: 130003741990
• NII Article ID: 10004866021
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.62.380
• COI: 1:CAS:528:DyaK28XjvFyrtLo%3D
• NDL BIB ID: 3978832
• ISSN: 09199268
• ISSN: http://id.crossref.org/issn/09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3978832
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/62/3/62_3_380/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed