Non-destructive Phosphorus-31 Nuclear Magnetic Resonance Study of Postmortem Changes in Oyster Tissues

• Yokoyama Yoshihiro

  Interdisciplinary Research Institute of Environmental Sciences Institute of Bioscience, Mukogawa Women's University

• Azuma Yasuyoshi

  Interdisciplinary Research Institute of Environmental Sciences

• Sakaguchi Morihiko

  Department of Fisheries, Faculty of Agriculture, Kyoto University

• Kawai Fumio

  Interdisciplinary Research Institute of Environmental Sciences

• Kanamori Masao

  Interdisciplinary Research Institute of Environmental Sciences

書誌事項

タイトル別名

• Non-destructive Phosphorus-31 Nuclear M

抄録

The postmortem changes of high energy phosphate compounds, inorganic phosphate (Pi), and intracellular pH (pHi) in the oyster tissues were investigated using non-destructive phosphorus-31 nuclear magnetic resonance (³¹P NMR) spectroscopy with a surface coil attachment. Levels of arginine phosphate (PAr), ATP, and ADP decreased faster in the adductor muscle than in the body trunk. Initial pHi in the adductor muscle, about 7.33, was significantly lower than that in the body trunk, about 7.48, although the two tissues had the same final pHi. Phosphodiesters (PDE) mainly composed of glycerophosphorylcholine were detected in both the tissues. ³¹P NMR was also used to evaluate the degree of freshness of oyster stored over a 25h period postmortem. The ratios of [PAr]/[Pi(+PDE+phosphomonoester (PME)] and [ATP]/[Pi(+PDE+PME)] and the pHi decreased rapidly and continuously during the acceptable stage, and were demonstrated as potential indices to estimate the freshness of oyster. ³¹P NMR is a powerful tool for the evaluation of freshness, because of its non-destructive, convenient, rapid, and simultaneous determination of high energy phosphate compounds, Pi, and pHi in the oyster tissues.

収録刊行物

• Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ

  Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ 62 (3), 416-420, 1996

  公益社団法人 日本水産学会

キーワード

• oyster
• nuclear magnetic resonance
• energy metabolism
• glycerophosphorylcholine
• freshness

詳細情報

• CRID: 1390282679406776448
• NII論文ID: 130003741996
• NII論文ID: 10004866214
• NII書誌ID: AA10993718
• DOI: 10.2331/fishsci.62.416
• NDL書誌ID: 3978840
• ISSN: 09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3978840
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/62/3/62_3_416/_pdf
• 本文言語コード: en
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• 抄録ライセンスフラグ: 使用不可