Responsibility of Myosin Rod for the Dimer Formation of Myosin Heavy Chain through SS Bonding during Ice Storage of Carp Actomyosin

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  • Responsibility of Myosin Rod for the Di

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Abstract

To clarify the role of the head and the tail portions of myosin in the dimer formation of myosin heavy chain through SS bonding during ice storage of carp actomyosin, carp myofibril and myosin digested with α-chymotrypsin to S-1 and rod were stored in ice with 2 ppm of Cu2+ to enhance the oxidation of SH groups.<br> A newly produced major substance through SS bonding was estimated to be 260K inmolecular weight, which was double the size of rod (130K). In addition, this substance was confirmed to be composed of only rod by two-step SDS-PAGE. A dimer of S-1 was not observed at all during the ice storage. Furthermore, the dimer formation of rod was also observed in the purified myosin rod solution.<br> It was, therefore, concluded that the formation of the dimer of myosin heavy chain during ice storage of carp actomyosin and myosin is due to the oxidation of SH groups on myosin rod, not on the myosin head.

Journal

  • Fisheries science

    Fisheries science 62 (3), 473-477, 1996

    The Japanese Society of Fisheries Science

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