The Use of a Monoclonal Antibody for the Detection of Fungal Parasite,Pythium sp.,the Causative Organism of Red Rot Disease,in Seawater from Porphyra Cultivation Farms
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- Amano Hideomi
- Laboratory of Marine Biochemistry, Faculty of Bioresources, Mie University
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- Sakaguchi Kenichi
- Laboratory of Marine Biochemistry, Faculty of Bioresources, Mie University Agriculture, Forestry, and Fisheries Divison, Mie Prefectural Government
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- Maegawa Miyuki
- Laboratory of Phycology, Faculty of Bioresources, Mie University
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- Noda Hiroyuki
- Laboratory of Marine Biochemistry, Faculty of Bioresources, Mie University
書誌事項
- タイトル別名
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- The Use of a Monoclonal Antibody for the Detection of Fungal Parasite, <i>Pythium</i> sp., the Causative Organism of Red Rot Disease, in Seawater from <i>Porphyra</i> Cultivation Farms
- Use of a Monoclonal Antibody for the De
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A monoclonal antibody was prepared against specific strains of the fungal parasite, Pythium sp., the causative organism of red rot disease of Porphyra, and its effectiveness in the early detection of the fungal pathogen was assessed. The antibody recognized germ-tubes of zoospores from Aichi, Saga and Miyagi Prefecturesbut failed to identify non-germinating zoospores. The isotype of the antibody was IgG1. Based on chemical and enzymatic treatments, the antigenic determinant was thought to involve glycoproteins of the germ-tube cell wall. For the enumeration of zoospores in seawater from Porphyra cultivation farms, seawater was pre-filtrated by 40 μm nylon mesh and zoospores were concentrated on 3 μm membrane filters and incubated in glucose-glutamate medium for 8h at 20°C. Germinating zoo-spores were detected by epifluorescence microscopy using the indirect fluorescent antibody technique. During the Porphyra cultivation season in Tsu, Mie Prefecture, germinating zoospores were detected from a farm employing a pole cultivation system. Between January 7 and February 13, 1995, numbers ranged from 11 germinating zoospores/l (the detection limit) to 4695 zoospores/l on January 25. No germinating zoospores were detected in seawater from a cultivation farm using a floating-net system despite the fact that diseased Porphyra thalli were observed in this farm.
収録刊行物
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- Fisheries science
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Fisheries science 62 (4), 556-560, 1996
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001204430159232
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- NII論文ID
- 130003742024
- 10004866771
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- NII書誌ID
- AA10993718
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- NDL書誌ID
- 4014509
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- ISSN
- 09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可