Isolation and Amino Acid Sequence of a Polypeptide Toxin from the Sea Anemone Radianthus crispus
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- Shiomi Kazuo
- Department of Food Science and Technology, Tokyo University of Fisheries
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- Lin Xin-Yu
- Department of Food Science and Technology, Tokyo University of Fisheries
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- Nagashima Yuji
- Department of Food Science and Technology, Tokyo University of Fisheries
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- Ishida Masami
- Department of Food Science and Technology, Tokyo University of Fisheries
書誌事項
- タイトル別名
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- Isolation and Amino Acid Sequence of a Polypeptide Toxin from the Sea Anemone <i>Radianthus crispus</i>
- Isolation and Amino Acid Sequence of a
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The aqueous extract of the sea anemone Radianthus crispus was potently lethal tocrabs but had no toxicity in mice. A polypeptide toxin (named Re I) was easily isolated by gel filtration on Sephadex G-50 and reverse-phase HPLC on TSKgel ODS-80Ts. Its minimum lethal dose against crabs was estimated to be 7.3 μg/kg. The complete amino acid sequence of Rc I was determined by sequence analyses of S-carboxamidomethylated toxin and its enzymatic fragments. Rc I comprises 47 amino acid residues and is characterized by the abundance of Asx, Gly, and half-Cys, the absence of Ala, Met, and Tyr, and the presence of an unusual amino acid, hydroxyproline. As compared with the sequences of the known sea anemone toxins, Rc I is ananalogue of type 1 toxins, showing an especially high homology with Cp I (89%) and Cp II (81%) from the Caribbean sea anemone Condylactis passiflora.
収録刊行物
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- Fisheries science
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Fisheries science 62 (4), 629-633, 1996
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001204428312704
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- NII論文ID
- 130003742050
- 10004867027
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- NII書誌ID
- AA10993718
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- NDL書誌ID
- 4014523
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- ISSN
- 09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可