Development of Monoclonal Antibodies that Specifically React with a Fish Pathogen,Vibrio anguillarum serotype J-O-1

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  • Development of Monoclonal Antibodies that Specifically React with a Fish Pathogen, <i>Vibrio anguillarum</i> serotype J-O-1
  • Development of Monoclonal Antibodies th

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Five monoclonal antibodies (MAbs) against cell surface antigens of Vibrio anguillarum serotype J-O-1 were developed by a hybridoma technique. Three of the MAbs, VA19-1, VA19-2, and VA19-4, reacted only with serotype J-O-1. The remaining two MAbs, VA07-1 and VA07-2, showed cross-reaction with some other Vibrio species. MAbs VA19-1, VA19-2, and VA19-4 could react with heat treated (100°C for 1h) cells of V. anguillarum J-O-1. This indicated that the antigens recognized by these MAbs were stable molecules such as lipopolysaccharide. As almost all strains of V. anguillarum isolated from diseased ayu Plecoglossus altivelis in freshwater belong to serotype J-O-1, MAbs VA19-1, VA19-2, and VA19-4 are useful for rapid identification and direct detection of V. anguillarum J-O-1 in natural freshwater environments. Furthermore, MAbs VA19-1, VA19-2, and VA19-4 specifically reacted with V. anguillarum serotype O2 among ten Danish serotypes (O1_??_O10) and did notreact with V. ordalii. Thus, these MAbs are also useful for distinction between closely related species, V. anguillarum O2 and V. ordalii.

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