RFLP and VNTR Markers Isolated from the Genomic DNA of Rainbow Trout and Cytogenetic Mapping by Fluorescence <i>In Situ</i> Hybridization

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  • Sakamoto Takashi
    Department of Aquatic Biosciences, Tokyo University of Fisheries
  • Okamoto Nobuaki
    Department of Aquatic Biosciences, Tokyo University of Fisheries
  • Ishii Mamoru
    Department of Aquatic Biosciences, Tokyo University of Fisheries
  • Maita Masashi
    Department of Aquatic Biosciences, Tokyo University of Fisheries
  • Ikeda Yayoi
    Department of Aquatic Biosciences, Tokyo University of Fisheries

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  • RFLP and VNTR Markers Isolated from the Genomic DNA of Rainbow Trout and Cytogenetic Mapping by Fluorescence In Situ Hybridization
  • RFLP and VNTR Markers Isolated from the

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Abstract

To obtain restriction fragment length polymorphism (RFLP) and variable number of tandem repeat (VNTR) markers from the rainbow trout genome, we constructed a genomic cosmid library from the RTG-2 cell line. Cosmid DNAs were prepared from 25 clones and examined to detect DNA polymorphisms. Three conventional RFLPs and 5 VNTR markers were identified. One of the VNTR markers (RVF9301) consisted of repeat units of a minisatellite tandem repeat of 52 bp inlength. In a primary study for cytogenetic mapping, we succeeded in localizing these markers on rainbow trout chromo-somes by fluorescence in situ hybridization. Our results suggest these 8 clones will serve as useful genetic markers for linkage analysis of genetic traits in fisheries science.

Journal

  • Fisheries science

    Fisheries science 62 (5), 719-722, 1996

    The Japanese Society of Fisheries Science

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