Cloning and Sequencing of Rainbow Trout β-Globin cDNA

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  • Yoshizaki Goro
    Department of Aquatic Biosciences, Tokyo University of Fisheries
  • Kang Jung-Ha
    Department of Aquatic Biosciences, Tokyo University of Fisheries
  • Sakuma Kazuko
    Department of Aquatic Biosciences, Tokyo University of Fisheries Japan NUS, Ltd.
  • Aoki Takashi
    Department of Aquatic Biosciences, Tokyo University of Fisheries
  • Takashima Fumio
    Department of Aquatic Biosciences, Tokyo University of Fisheries

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Other Title
  • Cloning and Sequencing of Rainbow Trout
  • Cloning and Sequencing of Rainbow Trout β-Globin cDNA

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Abstract

β-Globin cDNA was cloned from a total blood cell cDNA library using carp β-globin cDNA as the probe. This clone contained an open reading frame of 441 bp that encodes a polypeptide of 147 amino acids. The deduced amino acid sequence showed high identity with teleostei β-globin. The functionally important residues that are involved in the contacts with the heme or α-globin chain were shown to be highly conserved among all vertebrate β-globin. The structural features and homology analysis indicated that this molecule was an isoform of rainbow trout β-globin IV. Southern blot analysis using Hind III-digested rainbow trout genomic DNA showed multiple bands and restriction fragment length polymorphism was observed. These results suggest that rainbow trout possesses multiple β-globin gene family.

Journal

  • Fisheries science

    Fisheries science 62 (5), 723-726, 1996

    The Japanese Society of Fisheries Science

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