Flow Cytometry for DNA Contents of Somatic Cells and Spermatozoa in the Progeny of Natural Tetraploid Loach
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- Zhang Quanqi
- Faculty of Applied Biological Science, Hiroshima University
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- Arai Katsutoshi
- Faculty of Applied Biological Science, Hiroshima University
書誌事項
- タイトル別名
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- Flow Cytometry for DNA Contents of Soma
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Flow-cytometric assays were made for DNA contents of erythrocytic and other somatic cells in the progeny of natural tetraploids (4 n) of loach Misgurnus anguillicaudatus. When relative DNA contents of normal diploid loach are expressed as 2 C, the progeny produced from various fertilizations of gametes from diploids and tetraploids as well as chromosome manipulations such as duplication of maternalchromosomes and gynogenesis showed DNA contents which were theoretically expected.<br> Absolute DNA contents measured on the basis of chicken erythrocytic DNA for diploid, triploid, tetraploid, pentaploid and hexaploid loach were 2.53, 3.82, 5.10, 6.37, and 7.63 pg/nucleus, respectively. Therefore, there is a proportional relationship between the DNA contents and the ploidy status in the loach.<br> The relative DNA contents of the progeny of triploid loach confirmed that the triploid females laid both large triploid eggs and small haploid eggs. Those of the progeny of pentaploid loach demonstrated that pentaploid female laid only diploid eggs.<br> Active formation of 1 C (haploid), 2 C (diploid), and 3 C (triploid) spermatozoa was detected in the testes of diploid, tetraploid, and hexaploid males, respectively. In contrast, no evidence of maturation division of spermatogenesis was flow-cytometrically obtained in the triploid males examined. Pentaploid males gave a small peak at 2.3 C DNA contents, suggesting the formation of aneuploid spermatozoa.
収録刊行物
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- Fisheries science
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Fisheries science 62 (6), 870-877, 1996
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001204430001152
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- NII論文ID
- 130003902945
- 10004867965
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- NII書誌ID
- AA10993718
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- COI
- 1:CAS:528:DyaK2sXht1Ckt7s%3D
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- NDL書誌ID
- 4109746
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- ISSN
- 09199268
- http://id.crossref.org/issn/09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可