Flow Cytometry for DNA Contents of Somatic Cells and Spermatozoa in the Progeny of Natural Tetraploid Loach

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  • Flow Cytometry for DNA Contents of Soma

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Abstract

Flow-cytometric assays were made for DNA contents of erythrocytic and other somatic cells in the progeny of natural tetraploids (4 n) of loach Misgurnus anguillicaudatus. When relative DNA contents of normal diploid loach are expressed as 2 C, the progeny produced from various fertilizations of gametes from diploids and tetraploids as well as chromosome manipulations such as duplication of maternalchromosomes and gynogenesis showed DNA contents which were theoretically expected.<br> Absolute DNA contents measured on the basis of chicken erythrocytic DNA for diploid, triploid, tetraploid, pentaploid and hexaploid loach were 2.53, 3.82, 5.10, 6.37, and 7.63 pg/nucleus, respectively. Therefore, there is a proportional relationship between the DNA contents and the ploidy status in the loach.<br> The relative DNA contents of the progeny of triploid loach confirmed that the triploid females laid both large triploid eggs and small haploid eggs. Those of the progeny of pentaploid loach demonstrated that pentaploid female laid only diploid eggs.<br> Active formation of 1 C (haploid), 2 C (diploid), and 3 C (triploid) spermatozoa was detected in the testes of diploid, tetraploid, and hexaploid males, respectively. In contrast, no evidence of maturation division of spermatogenesis was flow-cytometrically obtained in the triploid males examined. Pentaploid males gave a small peak at 2.3 C DNA contents, suggesting the formation of aneuploid spermatozoa.

Journal

  • Fisheries science

    Fisheries science 62 (6), 870-877, 1996

    The Japanese Society of Fisheries Science

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