Identification of a cDNA for Medaka Cytoskeletal β-Actin and Construction for the Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) Primers
-
- Katagiri Takayuki
- Laboratory of Genetics and Biochemistry, Tokyo University of Fisheries
-
- Hirono Ikuo
- Laboratory of Genetics and Biochemistry, Tokyo University of Fisheries
-
- Aoki Takashi
- Laboratory of Genetics and Biochemistry, Tokyo University of Fisheries
書誌事項
- タイトル別名
-
- Identification of a cDNA for Medaka Cytoskeletal β-Actin and Construction for the Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) Primers
- Identification of a c DNA for Medaka Cy
この論文をさがす
抄録
A full-length cDNA for medaka cytoskeletal β-actin was isolated from a liver cDNA library. The entire coding region was 1125 bp long and was well conserved with respect to other vertebrate β-actins. Two primers for a reverse transcriptase-polymerase chain reaction (RT-PCR) assaywere constructed from the nonvariable region among medaka, carp, chicken, and mouse β-actin. RT-PCR amplified a 510 bp DNA band from medaka cDNA synthesized from the blood, brain, heart, intestine, kidney, liver, muscle, ovary, spleen, and testis poly(A)+mRNA.<br> The constructed primers were used successfully to detect a specific 510 bp fragment of β-actin from zebrafish, rainbow trout, and Japanese flounder.
収録刊行物
-
- Fisheries science
-
Fisheries science 63 (1), 73-76, 1997
公益社団法人 日本水産学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204428495360
-
- NII論文ID
- 130003902993
- 10004868688
-
- NII書誌ID
- AA10993718
-
- NDL書誌ID
- 4153990
-
- ISSN
- 09199268
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可