Purification and Characterization of Chitinase from the Liver of Japanese Common Squid Todarodes pacificus

• Matsumiya Masahiro

  College of Bioresource Sciences, Nihon University

• Mochizuki Atsushi

  College of Bioresource Sciences, Nihon University

書誌事項

タイトル別名

• Purification and Characterization of Chitinase from the Liver of Japanese Common Squid <i>Todarodes pacificus</i>
• Purification and Characterization of Ch

抄録

A chitinase (EC 3. 2. 1. 14) was purified from the liver of Japanese common squid Todarodes pacificus by ammonium sulfate fractionation and column chromatographies with Chitopearl Basic BL-03, CM-Toyopearl 650 S, and Bio-Gel HTP. The purified enzyme gave a single protein band on polyacrylamide gel electrophoresis (PAGE), and its molecular weight was estimated tobe 38 kDa by SDS-PAGE. The isoelectric point was 8. 3. The enzyme showed two optimum pHs at 1.5 and 8.5 using glycol chitin as the substrate. The enzyme was stable between pH 4.0 and 6.0 after incubation at 50°C for 10 min. The optimum temperature was 50°C. The chitinase hydrolyzed glycol chitin and colloidal chitin, but not p-nitrophenyl N-acetyl-β-D-glucosaminide and Micrococcus lysodeikticus. The cleavage pattern was investigated using N-acetylchitooligosaccharides (GlcNAc_n, n=2 to 6). The enzyme hydrolyzed GlcNAc₄ to two molecules of GlcNAc₂, GlcNAc₅ to GlcNAc₂ plus GlcNAc₃, and GlcNAc₆ to GlcNAc2 plus GlcNAc₄ (84%) and two molecules of GlcNAc₃ (16%). The substrate specificityof the enzyme was that of endo-type chitinase.

収録刊行物

• Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ

  Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ 63 (3), 409-413, 1997

  公益社団法人 日本水産学会

キーワード

• chitinase
• purification
• characterization
• liver
• Japanese common squid
• Todarodes pacificus
• substrate specificity
• cleavage pattern

詳細情報

• CRID: 1390001204429346688
• NII論文ID: 130003903030
• NII論文ID: 10004870158
• NII書誌ID: AA10993718
• DOI: 10.2331/fishsci.63.409
• COI: 1:CAS:528:DyaK2sXjvFaitLo%3D
• NDL書誌ID: 4231327
• ISSN: 09199268
• ISSN: http://id.crossref.org/issn/09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4231327
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/63/3/63_3_409/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可