Isolation and Properties of a Sialoglycoprotein from the Skin Mucus of the Stingray Dasyatis akajei

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  • Sumi Toshihisa
    Department of Applied Biological Sciences, Faculty of Agriculture, Saga University
  • Hama Yoichiro
    Department of Applied Biological Sciences, Faculty of Agriculture, Saga University
  • Maruyama Daisuke
    Department of Applied Biological Sciences, Faculty of Agriculture, Saga University
  • Asakawa Makio
    Laboratory of Food Science, Faculty of Education, Kumamoto University
  • Nakagawa Hiroki
    Department of Applied Biological Sciences, Faculty of Agriculture, Saga University

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  • Isolation and Properties of a Sialoglycoprotein from the Skin Mucus of the Stingray <i>Dasyatis akajei</i>
  • Isolation and Properties of a Sialoglyc

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A sialoglycoprotein (SGP) has been purified from the skin mucus of the stingray Dasyatis akajei. SGP contained 22.1% (w/w) NeuAc, 24.4% GalNAc, 9.9% GlcNAc, 6.1% Gal, and 27% amino acids, and its average molecular weight (Mr) was estimated to be 500, 000. SGP was very rich in Thr (32 mol%) and Ser (12 mol%). Treatment of SGP with alkali (β-elimination of carbohydrate chains) result-ed in the destruction of 70% of Thr and Ser, indicating that the carbohydrate chains were attached through these amino acids. Exhaustive digestion of SGP by actinase yielded a highly glycosylated glycopeptide with Mr of 50, 000. Based on these results, it is assumed that a SGPmolecule bears more than 400 oligosaccharide chains which are attached to the Thr and Ser residues of the polypeptide backbone and spaced at an average of 3 amino acids apart.

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