Detection and Identification of Infectious Hematopoietic Necrosis Virus (IHNV) by Reverse Transcription (RT)-polymerase Chain Reaction (PCR)
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A method based on reverse transcription (RT)-polymerase chain reaction (PCR) was established for detection and identification of infectious hematopoietic necrosis virus (IHNV). A set of primers was prepared for amplification of a 510 bp nucleotide which encodes a region of IHNV nucleoprotein (N) gene. The PCR product was confirmed as the IHNV specific nucleotide by southern hybridization with an oligonucleotide probe synthesized from the N gene. The PCR was able to amplify the target sequence from five representative strains of IHNV from Japan and North America. There was no PCR product from five other fish rhabdoviruses. Viruses from ovarian fluid of masu salmon collected at Shibetsu River, Ichani River in Hokkaido and kidney tissue of rainbow trout in Aichi Prefecture were isolated and identified using the RT-PCR.
- Fisheries Science
Fisheries Science 63(4), 592-595, 1997
Japanese Society of Fisheries Science