Detection and Identification of Infectious Hematopoietic Necrosis Virus (IHNV) by Reverse Transcription (RT)-polymerase Chain Reaction (PCR)

• Yoshinaka Toko

  Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University

• Yoshimizu Mamoru

  Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University

• Sawabe Tomoo

  Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University

• Ezura Yoshio

  Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University

書誌事項

タイトル別名

• Detection and Identification of Infecti

抄録

A method based on reverse transcription (RT)-polymerase chain reaction (PCR) wasestablished for detection and identification of infectious hematopoietic necrosis virus (IHNV). A set of primers was prepared for amplification of a 510 bp nucleotide which encodes a region of IHNV nucleoprotein (N) gene. The PCR product was confirmed as the IHNV specific nucleotide by southern hybridization with an oligonucleotide probe synthesized from the N gene. The PCR was able to amplify the target sequence from five representative strains of IHNV from Japan and North America. There was no PCR product from five other fish rhabdoviruses. Viruses from ovarian fluid of masu salmon collected at Shibetsu River, Ichani River in Hokkaido and kidney tissue of rainbow trout in Aichi Prefecture were isolated and identified using the RT-PCR.

収録刊行物

• Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ

  Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ 63 (4), 592-595, 1997

  公益社団法人 日本水産学会

キーワード

• IHNV
• RT-PCR
• fish-rhabdoviruses
• rhabdovirus

詳細情報

• CRID: 1390001204428786944
• NII論文ID: 130003903068
• NII論文ID: 10004870891
• NII書誌ID: AA10993718
• DOI: 10.2331/fishsci.63.592
• HANDLE: 2115/52089
• NDL書誌ID: 4277910
• ISSN: 09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4277910
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/63/4/63_4_592/_pdf
• 本文言語コード: en
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