Aggregation of Actin and Myosin in Carp Myofibrils during Frozen Storage under Phosphate Buffer Conditions
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- Kitazawa Hiroyuki
- Faculty of Fisheries, Hokkaido University
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- Kawai Yuji
- Faculty of Fisheries, Hokkaido University
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- Yamazaki Koji
- Faculty of Fisheries, Hokkaido University
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- Inoue Norio
- Faculty of Fisheries, Hokkaido University
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- Shinano Haruo
- Faculty of Fisheries, Hokkaido University
Bibliographic Information
- Other Title
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- Aggregation of Actin and Myosin in Carp
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Abstract
Carp myofibrils suspended in 5 and 50mM phosphate buffer (pH 6.8) were stored at -4, -11, and -26°C for 167 days, and the changes in volume of myofibrillar protein components in salt-soluble and salt-insoluble fractions were followed by polyacrylamide gel electrophoresis. The extents of the decrease of actin and myosin heavy chain components in the salt-soluble fraction and the increase of those in the salt-insoluble fraction were greater, and the insolubilization of those components was accelerated with an increase in storage temperature at both buffer concentrations. Under all storage conditions, there was a transient steady stage during which the changes of actin and myosin heavy chain components were very small in each fraction. The steady stages in 5 and 50mM phosphate buffers occurred at storage periods 10-89 (or 10-40) and 40-89, respectively, and especially those of the actin component in the salt-insoluble fraction were evident. The beginning of the steady stage in 5mM phosphate buffer was earlier than that in 50mM. It seems that the myofibrils were insolubilized as the actin-myosin complex in the storage range of 0-23 days at -4°C in 5mM phosphate buffer and in the range of 0-89 days under all other storage conditions.
Journal
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- Fisheries science
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Fisheries science 63 (4), 635-638, 1997
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390001204428789376
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- NII Article ID
- 130003903073
- 10004871044
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- NII Book ID
- AA10993718
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- NDL BIB ID
- 4277919
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- ISSN
- 09199268
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed