Analysis of <i>Gymnodinium catenatum</i> Dinophyceae Using Sequences of the 5.8S rDNA-ITS Regions and Random Amplified Polymorphic DNA

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  • Adachi Masao
    Laboratory of Aquatic Environmental Science, Department of Aquaculture, Faculty of Agriculture, Kochi University
  • Sako Yoshihiko
    Laboratory of Marine Microbiology, Department of Applied Bioscience, Graduate School of Agriculture, Kyoto University
  • Ishida Yuzaburo
    Faculty of Science and Engineering, Ritsumeikan University

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Other Title
  • Analysis of Gymnodinium catenatum Dinophyceae Using Sequences of the 5.8S rDNA-ITS Regions and Random Amplified Polymorphic DNA
  • Analysis of Gymnodinium catenatum Dinop

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Abstract

The genetic relatedness of the toxigenic dinoflagellate Gymnodinium catenatum Graham from Australia, Japan, Portugal and Spain were examined using sequence comparison of the 5.8 S rDNA and internal transcribed spacer (ITS) regions and/or random amplified polymorphic DNA (RAPD) analysis. The sequence analysis showed that the sizes of ITSI, ITS2 and the 5.8 S rDNA in six isolates of G. catenatum were conserved and were 192, 223 and 160 bp, respectively. ITS and the 5.8 S rDNA regions between all isolates tested were found to be extremely homogeneous and were divergent from several other dinoflagellates. Aligned nucleotide sequences in the 5.8 S rDNA region of G. catenatum and several species of dinoflagellates showed the existence of DNA signatures (base pair 90-115) specific to G. catenatum in this region. Therefore, the nuclear ITS spacer regions of G. catenatum provide phylo-genetically informative and G. catenatum-specific genetic markers. RAPD patternsrevealed the subdivided population of G. catenatum. The isolate from Japan (JP02) exhibited almost the same RAPD patterns as those of the two isolates from Portugal (PR01) and Spain (GC21V) and was clearly discriminated from the four isolates from Australia. Two isolates from Australia (DE06 and DE08) showed similar RAPD patterns. Three other isolates (GPU2, HU02, and HU07) exhibited unique RAPD patterns. This is the first report about the application of RAPDs to discriminate dinoflagellates. RAPD may provide a useful and rapid tool for the genetic differentiation of these organisms at intra-species level.

Journal

  • Fisheries science

    Fisheries science 63 (5), 701-707, 1997

    The Japanese Society of Fisheries Science

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