Pharmacological Dose of Estradiol-17β Induces Vitellogenin Synthesis in Cultured Hepatocytes of Immature Eel Anguilla japonica

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  • Komatsut Masaharu
    Laboratory of Food Chemistry, Faculty of Fisheries, Kagoshima University Masaharu Komatsu, Ph. D. The Institute fro Cancer Research, Faculty of Medicine, Kagoshima University
  • Hayashi Seiichi
    Laboratory of Food Chemistry, Faculty of Fisheries, Kagoshima University

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タイトル別名
  • Pharmacological Dose of Estradiol-17β Induces Vitellogenin Synthesis in Cultured Hepatocytes of Immature Eel <i>Anguilla japonica</i>
  • Pharmacological Dose of Estradiol-17ベータ
  • Pharmacological Dose of Estradiol-17&beta; Induces Vitellogenin Synthesis in Cultured Hepatocytes of Immature Eel <i>Anguilla japonica</i>

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Synthesis and secretion of the protein with molecular weight 196 K on SDS-polyacrylamide gel electrophoresis was induced in immature Japanese eel hepatocytes by pharmacologically high concentration of estradiol-17β (E2), 10-4M, during 4 days culture, but it was not induced by 10-5M and 10-6M E2 during the same or more days culture. The protein with the same molecular weight was observed in the plasma of the eel into which E2 was injected intraperitoneally.<br> Vitellogenin, a very-high-density lipoprotein with density in the range of 1.27 to 1.30g/ml, was isolated from the plasma of female silver eel and also from that of E2 treated immature eels by density gradient ultracentrifugation. This lipoprotein was further purified by a DEAE-Toyopearl column and characterized to consist of a major apolipoprotein with 196 K. These results in dicate that the 196 K protein that appeared in the culture medium of hepatocytes is the apolipoprotein of vitellogenin.<br> Hepatocytes prepared from the preliminarily E2-injected eel secreted the vitellogenin after 3 days culture with 10-6M E2, but did not secrete the vitellogenin after the same or more days culture without E2.

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