Sequence and Expression of a cDNA Encoding the Red Seabream Estrogen Receptor

• Touhata Ken

  Advanced Course of Applied Biosciences, Graduate School of Agriculture, Kyoto University

• Kinoshita Masato

  Advanced Course of Applied Biosciences, Graduate School of Agriculture, Kyoto University

• Toyohara Haruhiko

  Advanced Course of Applied Biosciences, Graduate School of Agriculture, Kyoto University

• Sakaguchi Morihiko

  Advanced Course of Applied Biosciences, Graduate School of Agriculture, Kyoto University

Bibliographic Information

Other Title

• Sequence and Expression of a c DNA Enco

Abstract

The cDNA of the estrogen receptor (ER) has been isolated from red seabream liver and sequenced. The cDNA contains a complete open reading frame encoding 581 amino acid residues. The structural homology indicated that the red seabream ER (rsER) lacks region A in region A/B (trans-activation domain) as well as tilapia, medaka and rainbow trout ERs. In region B, amino acids were conserved also in rsER around a serine residue that is putatively phosphorylated by a mitogen-activated protein kinase. In region C (DNA binding domain), rsER shows a high degree of sequence conservation with other fish ERs, particularly eight cysteins in two zinc-fingers and the P-box were completely conserved. Region E (hormone binding domain) was also highly conserved. On the other hand, in regions D and F, low homology was revealed.<br> The trans-activation function of this clone was verified by transfection into HeLa cells, which lack endogenous ER. A CMV-based expression vector consisting of rsER cDNA was cotransfected with a chimeric reporter plasmid consisting of an estrogen responsive element fused to the thymidine kinase promoter and the chloramphenicol acetyl transferase (CAT) gene. In the presence of estradiol 17-β, the CAT activity was stimulated by up to 10-fold. This showed that this clone encoded the functional receptor.

Journal

• Fisheries science

  Fisheries science 64 (1), 131-135, 1998

  The Japanese Society of Fisheries Science

Keywords

• estrogen
• estrogen receptor
• red seabream
• <i>trans</i>-activation function

Details

• CRID: 1390001204429805440
• NII Article ID: 130003742117
• NII Article ID: 10004873336
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.64.131
• COI: 1:CAS:528:DyaK1cXhtlygtLo%3D
• NDL BIB ID: 4406516
• ISSN: 09199268
• ISSN: http://id.crossref.org/issn/09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4406516
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/64/1/64_1_131/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed