Presence of Cysteine Endopeptidase in the Pyloric Caeca of Pacific Mackerel Scomber japonicus and Its Purification and Characterization
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- Tsukamasa Yasuyuki
- Department of Fisheries, Faculty of Agriculture, Kinki University
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- Nakagawa Takayuki
- Department of Fisheries, Faculty of Agriculture, Kinki University
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- Masuda Kazuhiro
- Department of Fisheries, Faculty of Agriculture, Kinki University
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- Ando Masashi
- Department of Fisheries, Faculty of Agriculture, Kinki University
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- Makinodan Yasuo
- Department of Fisheries, Faculty of Agriculture, Kinki University
書誌事項
- タイトル別名
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- Presence of Cysteine Endopeptidase in the Pyloric Caeca of Pacific Mackerel <i>Scomber japonicus</i> and Its Purification and Characterization
- Presence of Cysteine Endopeptidase in t
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An endopeptidase which hydrolyzed myofibril strongly from the pyloric caeca of Pacific mackerel Scomber japonicus was purified to homogeneity electrophoretically. The molecular weight and the optimum pH of the endopeptidase was about 30k and around 9.5, respectively. Since the endopeptidase was not inhibited by serine protease inhibitors, e. g. PMSF and soybean trypsin inhibitor, but completely inhibited by E-64, a high specific cysteine protease inhibitor, it was considered to be a cysteine endopeptidase. This is the first report on the existence of the cysteine endopeptidase in the pyloric caeca. The endopeptidase showed a hydrolytic specificity for MCA-synthetic peptide substrates which had a basic amino acid at P1 position and a hydrophobic amino acid at P2 or P3 position. It hydrolyzed various proteins examined except for collagen.
収録刊行物
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- Fisheries science
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Fisheries science 64 (4), 627-632, 1998
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390282679404775936
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- NII論文ID
- 130003903214
- 10004875601
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- NII書誌ID
- AA10993718
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- NDL書誌ID
- 4547685
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- ISSN
- 09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可