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- Hashimoto Hisashi
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University Japan Society for the Promotion of Science
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- Matsuo Yoshiyuki
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- Yokoyama Yoshihiro
- Interdisciplinary Research Institute of Bioscience, Mukogawa Women's University
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- Toyohara Haruhiko
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- Sakaguchi Morihiko
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
書誌事項
- タイトル別名
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- Induction of Apoptosis in Fish Cells by
この論文をさがす
抄録
Effects of osmotic stresses on apoptotic cell death of a fish cell line (Epithelioma Papulosum Cyprini, EPC) were investigated. EPC showed DNA fragmentation, which is a biochemical feature of apoptosis, under hypertonic stress, when exposed to 400-600mOsm/kg media with sodium chloride supplementation. Similar results were obtained upon exposure to 450mOsm/kg medium with sorbitol. DNA fragmentation increased significantly within 3h after exposure to the hypertonic stress. Nuclear condensation, which is a morphological hallmark of apoptosis, was also observed in the culture of EPC exposed to hypertonic stress. The amount of native nucleosomal DNA was evaluated to find whether the whole cell population undergoes apoptosis. As a result, hypertonicity below 500mOsm/kg triggered apoptotic cell death only in a part of the whole cell population, while 600mOsm/kg brought about cell death in a large proportion of the population by necrosis as well as apoptosis. In contrast, hypotonic media (150 and 200mOsm/kg) did not induce DNA fragmentation. DNA fragmentation of EPC induced by hypertonic stress was suppressed in the presence of Zn2+, suggesting that a Zn2+-sus-ceptible endonuclease(s) may be responsible for cleavage of nucleosomal DNA.
収録刊行物
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- Fisheries science
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Fisheries science 64 (5), 820-825, 1998
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390282679406550400
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- NII論文ID
- 130003903249
- 10004876395
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- NII書誌ID
- AA10993718
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- NDL書誌ID
- 4591603
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- ISSN
- 09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可