Differential Expression of mRNAs in the Marine Diatom <i>Chaetoceros compressum</i> Exposed to High Temperatures

• Kinoshita Shigeharu

  Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Kikuchi Kiyoshi

  Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Yamada Hiroshi

  Central Laboratory Marine Ecology Research Institute

• Hara Takeya

  Central Laboratory Marine Ecology Research Institute

• Itoh Yasuo

  Central Laboratory Marine Ecology Research Institute

• Kinoshita Hideaki

  Central Laboratory Marine Ecology Research Institute

• Watabe Shugo

  Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Bibliographic Information

Other Title

• Differential Expression of mRNAs in the Marine Diatom Chaetoceros compressum Exposed to High Temperatures
• Differential Expression of mRNA s in th

Abstract

We investigated mRNA expression patterns in the laboratory-reared diatom Chaetoceros compressum under temperature stress conditions. The diatom incubated at 30°C for 15min, showing 43% growth rate in a subsequent culture at 20°C for 2 days, were analyzed by mRNA arbitrarily primed reverse transcription-polymerase chain reaction (RAP RT-PCR) in comparison with control cells incubated at 20°C throughout. The heat stress at 30°C induced expression changes in 7 cDNAs, one of which showed 37% identity to a homologous region of the Bacteroides fragilis glutamine synthetase gene. This stress on the diatom resulted in 2.5-fold increase in its mRNA levels. Our results demonstrated that the gene (s) related to this cDNA is expressed in response to heat stress for the diatom, suggesting its possible applications to monitoring marine environments.

Journal

• Fisheries science

  Fisheries science 64 (5), 831-835, 1998

  The Japanese Society of Fisheries Science

Keywords

• diatom
• Chaetoceros compressum
• heat stress
• hsps
• RAP RT-PCR
• glutamine
• glutamine synthetase

Details

• CRID: 1390282679406555008
• NII Article ID: 130003903251
• NII Article ID: 10004876456
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.64.831
• NDL BIB ID: 4591605
• ISSN: 09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4591605
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/64/5/64_5_831/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed