Fish Mucus Stimurates the Generation of Superoxide Anion by <i>Chattonella marina</i> and <i>Heterosigma akashiwo</i>

• Nakamura Atsushi

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Okamoto Tarou

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Komatsu Nobukazu

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Ooka Seiji

  Marine Research Institute, Nagasaki University

• Oda Tatsuya

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Ishimatsu Atsushi

  Marine Research Institute, Nagasaki University

• Muramatsu Tsuyoshi

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

Bibliographic Information

Other Title

• Fish Mucus Stimurates the Generation of Superoxide Anion by Chattonella marina and Heterosigma akashiwo
• Fish mucus stimulates the generation of superoxide anion by Chattonella marina and Heterosigma akashiwo
• Fish mucus stimulates the generation of superoxide anion by Chattonella antiqua and Heterosigma akashiwo

Abstract

Chattonella marina and Heterosigma akashiwo are highly toxic red tide phytoplankton which causes severe damage to fish farming. Recent studies demonstrated that these flagellates release superoxide anion (O₂^-) under normal growth conditions. In the present study, we examined the effects of the fish mucus prepared from skin and gill of yellowtail, red sea bream, and japanese flounder on the production of ₂^- by the flagellates. All mucus tested stimulated C. marina and H. akashiwo to generate increased amounts of O₂^- in a concentration dependent manner. Thus, the common substances present in fish mucus of three species may be responsible for the stimulation of the flagellates. In the presence of 100μg/ml of each mucus, 4-6-fold increased level of O₂^- was detected in both flagellates. After the heat treatment (at 121°C for 15min), no significant decrease of stimulatory effects of the mucus was observed, suggesting that heat-stable components of the mucus may produce the stimulatory effect on the flagellates. Our results suggest the possibility that the fish mucus enhances the oxygen radical-mediated toxic effects of C. marina and H. akashiwo.

Journal

• Fisheries science

  Fisheries science 64 (6), 866-869, 1998

  The Japanese Society of Fisheries Science

Keywords

• red tide plankton
• Raphidophyceae
• fish mucus
• reactive oxygen species

Details

• CRID: 1390282679405067136
• NII Article ID: 130003903262
• NII Article ID: 10004876624
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.64.866
• COI: 1:CAS:528:DyaK1MXjs1ymug%3D%3D
• NDL BIB ID: 106919
• ISSN: 09199268
• ISSN: http://id.crossref.org/issn/09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I106919
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/64/6/64_6_866/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed