Comparison of Partial Nucleotide Sequences in the Exonic Region of a Small Subunit Ribosomal RNA Gene for Discrimination of Laver (<i>Porphyra</i>) Species and Cultivars

• Mizukami Yuzuru

  Department of Applied Aquabiology, National Fisheries University

• Kaminishi Yoshio

  Department of Food Science and Technology, National Fisheries University

• Kunimoto Masahiko

  Department of Food Science and Technology, National Fisheries University

• Kobayashi Masahiro

  Ishigaki Tropical Station, Seikai National Fisheries Research Institute

• Murase Noboru

  Department of Applied Aquabiology, National Fisheries University

• Kito Hitoshi

  Department of Applied Aquabiology, National Fisheries University

Bibliographic Information

Other Title

• Comparison of Partial Nucleotide Sequences in the Exonic Region of a Small Subunit Ribosomal RNA Gene for Discrimination of Laver(Porphyra)Species and Cultivars

Abstract

Partial nucleotide sequences in the exonic region of a small subunit ribosomal RNA gene (ssr DNA) were analysed for nine commercially utilized species of Porphyra and eight laver cultivars in order to examine whether these sequences can be used to identify and discriminate between these species and cultivars. Pairwise comparisons of a total of 555 nucleotides revealed that there were from one to ninety-one nucleotide differences (substitutions and insertions/deletions) among the nine species. The one nucleotide difference found between Porphyrayezoensis and P. tenera was authentic, confirmed by repetitive sequence analyses of ssr DNA exonic regions using thalli of these species collected from five different locations. The nucleotide sequences of eight laver cultivars were identical to each other and also identical to field-collected P. yezoensis but not P. tenera. These results suggest that the nine Porphyra species can be distinguished from each other by comparison of the partial nucleotide sequences of the ssr DNA exonic region, while discrimination of laver cultivars is not possible by the comparison of only these sequences.

Journal

• Fisheries science

  Fisheries science 64 (6), 886-891, 1998

  The Japanese Society of Fisheries Science

Keywords

• Porphyra yezoensis
• Porphyra tenera
• red algae
• small subunit ribosomal RNA gene sequences
• laver cultivars
• species identification

Details

• CRID: 1390282679405062144
• NII Article ID: 130003903264
• NII Article ID: 10004876713
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.64.886
• NDL BIB ID: 106948
• ISSN: 09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I106948
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/64/6/64_6_886/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed