An Accessory Protein of the Iron-regulated Hemolysin of Edwardsiella tarda is Necessary for Hemolytic Activity

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著者

    • Hirono Ikuo HIRONO Ikuo
    • Laboratory of Genetics and Biochemistry, Department of Aquatic Biosciences, Tokyo University of Fisheries

抄録

The eth hemolysin gene locus of Edwardsiella tarda encodes the hemolysin EthA protein and its accessory protein EthB. We constructed mutants by site directed marker insertion mutagenesis in ethA or ethB by a method employing the integration of a mobilizable suicide plasmid containing a portion of the ethA or ethB coding sequence into the chromosome. Both the ethA- and ethB-matants lacked extracellular, cell-associated, and intracellular hemolytic activities. These results indicate that the EthB protein is essential for the hemolytic activity of EthA. The EthA protein may be activated in the periplasmic region of the cell by the EthB protein which is located on the outer membrane of the cell. The loss of hemolytic activity of the ethA- and ethB-deficient mutants suggests that E. tarda has only one hemolysin gene locus in the genome.

収録刊行物

  • Fisheries science : FS  

    Fisheries science : FS 64(6), 924-928, 1998-12-01 

    公益社団法人 日本水産学会

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各種コード

  • NII論文ID(NAID)
    10004876888
  • NII書誌ID(NCID)
    AA10993718
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09199268
  • NDL 記事登録ID
    107105
  • NDL 雑誌分類
    ZR26(科学技術--農林水産--水産)
  • NDL 請求記号
    Z54-H592
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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