Production of β-<i>N</i>-Acetylglucosaminidase and Chitinase by <i>Aeromonas</i> Species Isolated from River Fish

• Sugita Haruo

  Department of Marine Science and Resources, Nihon University

• Yamada Shogo

  Department of Marine Science and Resources, Nihon University

• Konagaya Yukifumi

  Department of Marine Science and Resources, Nihon University Department of Food Science and Technology, Tokyo University of Fisheries

• Deguchi Yoshiaki

  Department of Marine Science and Resources, Nihon University

Bibliographic Information

Other Title

• Production of β-N-Acetylglucosaminidase and Chitinase by Aeromonas Species Isolated from River Fish
• Production of ベータ N Acetylglucosaminidase and Chitinase by Aeromonas Species Isolated from River Fish
• Production of &beta;-<i>N</i>-Acetylglucosaminidase and Chitinase by <i>Aeromonas</i> Species Isolated from River Fish

Abstract

The β-N-acetylglucosaminidase (β-GlcNAcase)-and chitinase-producing abilities of 283 strains in Aeromonas species from intestinal tracts of common carp Cyprinus carpio, crucian carp Carassius auratus, and gray mullet Mugil cephalus, and from their environment were determined using three fluorogenic 4-methylumbelliferone glycosides of N-acetylglucosamine oligosaccharides. As many as 91 to 100% of strains in each Aeromonas species produced at least one of three enzymes, such as β-GlcNA-case, exo-chitinase and endo-chitinase, and 58 to 80% of strains could produce all three enzymes. Enzyme activities varied remarkably with species and strains, along with bacterial sources, and 20 to 55% of A. hydrophila (except for β-GlcNAcase), A. jandaei (except for β-GlcNAcase) and A. sobria showed high activities (>0.1U/mg) of β-GlcNAcase and chitinase. Moreover, there was a significant strain-to-strain variation in composition of chitinoclastic enzymes even within genospecies. These results suggest that the predominant aeromonads in the intestine of freshwater fish play an important role in the digestion of injested chitin-bearing organisms.

Journal

• Fisheries science

  Fisheries science 65 (1), 155-158, 1999

  The Japanese Society of Fisheries Science

Keywords

• chitinase
• β-<i>N</i>-acetylglucosaminidase
• <i>Aeromonas</i> spp.
• intestinal bacteria
• fluorogenic substrate
• river fish

Details

• CRID: 1390001204429680512
• NII Article ID: 130003903325
• NII Article ID: 10004877864
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.65.155
• NDL BIB ID: 4666301
• ISSN: 09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4666301
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/65/1/65_1_155/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed